Acute seizure suppression by transcranial direct current stimulation in rats
نویسندگان
چکیده
OBJECTIVE Cathodal transcranial direct current stimulation (tDCS) is a focal neuromodulation technique that suppresses cortical excitability by low-amplitude constant electrical current, and may have an antiepileptic effect. Yet, tDCS has not been tested in status epilepticus (SE). Furthermore, a combined tDCS and pharmacotherapy antiseizure approach is unexplored. We therefore examined in the rat pentylenetetrazol (PTZ) SE model whether cathodal tDCS (1) suppresses seizures, (2) augments lorazepam (LZP) efficacy, and (3) enhances GABAergic cortical inhibition. METHODS Experiment 1 aimed to identify an effective cathodal tDCS intensity. Rats received intraperitoneal PTZ followed by tDCS (sham, cathodal 1 mA, or cathodal 0.1 mA; for 20 min), and then a second PTZ challenge. In Experiment 2, two additional animal groups received a subtherapeutic LZP dose after PTZ, and then verum or sham tDCS. Clinical and electroencephalography (EEG) epileptic activity were compared between all groups. In Experiment 3, we measured GABA-mediated paired-pulse inhibition of the motor evoked potential by paired-pulse transcranial magnetic stimulation (ppTMS) in rats that received PTZ or saline, and either verum or sham tDCS. RESULTS Cathodal 1 mA tDCS (1) reduced EEG spike bursts, and suppressed clinical seizures after the second PTZ challenge, (2) in combination with LZP was more effective in seizure suppression and improved the clinical seizure outcomes compared to either tDCS or LZP alone, and (3) prevented the loss of ppTMS motor cortex inhibition that accompanied PTZ injection. INTERPRETATION These results suggest that cathodal 1 mA tDCS alone and in combination with LZP can suppress seizures by augmenting GABAergic cortical inhibition.
منابع مشابه
Effect of different frequencies of repetitive transcranial magnetic stimulation (rTMS) on acquisition of chemical kindling seizures in rats
IIntroduction: Repetitive transcranial magnetic stimulation (rTMS) modulates the excitability of cortical neural networks. The effect of rTMS on excitability of cortical networks depends on its frequency. According to the previous reports, a distinction is made between low (<1Hz) and high frequencies of rTMS. Low frequencies of rTMS inhibit seizure but high frequencies increase it. In the curre...
متن کاملThe role of adenosine A1 receptors on post seizure depression period in rats
Epilepsy is among the most common disorders of the central nervous system and there is not an absolute method for its treatment. It has been shown that each seizure has a depressing effect on the following seizure. Thus, finding the mechanisms responsible in this phenomenon can improve our knowledge toward new ways for epilepsy treatment. In this study, the role of adenosine A1 receptors in ...
متن کاملEffect of Direct Transcranial Current Stimulation on Pain Intensity of Burn Dressing
Background: Pain is of stressful and unpleasant procedures after daily dressing of burn wound, so that if the burn pain is not alleviated properly, it can cause harmful effects other than discomfort. There is evidence that cathodal stimulation of somatosensory cortex can be effective in relieving acute pain. Aim: The main purpose of this study was to determine the effect of Direct Transcranial ...
متن کاملAttention-deficit/Hyperactivity, sustained attention, response inhibition, Mindfulness-based socio-emotional Learning, Transcranial Direct Current Stimulation.
This research aimed to compare the effectiveness of mindfulness-based socio-emotional learning program with/and without Transcranial Direct Current Stimulation on sustained attention and response inhibition in children with attention deficit/hyperactivity disorder. This research was a semi-experimental study with a pre-test and post-test design with a control group. The statistical population i...
متن کامل